Chromosorb 101 是一种经典的气相色谱（GC）固定相，属于多孔聚合物微球（Porous Polymer Beads）类型。它是由约翰·曼维尔（Johns Manville）公司在其“Chromosorb”系列色谱载体中推出的产品之一。

基本性质与化学结构
化学组成：Chromosorb 101 是一种苯乙烯-二乙烯基苯（Styrene-Divinylbenzene）的共聚物。二乙烯基苯（DVB）作为交联剂，将线性的聚苯乙烯链连接成一个三维网络结构，从而形成具有机械强度和热稳定性的多孔微球。
极性：它是非极性的固定相，其分离机理主要基于范德华力和疏水相互作用。
物理形态：通常是白色或乳白色的微小颗粒，具有均匀的粒径（如80-100目，100-120目等），以便填充到色谱柱中。
表面特性：相比硅藻土类载体（如Chromosorb W），它的表面是惰性的，几乎没有活性吸附位点。这使得它特别适合分析极性化合物，如醇、酸、胺等，因为这些化合物在惰性表面上不易发生拖尾现象。

主要特点
惰性表面：*大优点。减少了分析极性样品（尤其是水、醇、酸）时的峰拖尾问题，能得到对称的色谱峰。
热稳定性好：*高使用温度通常可达 250°C 至 275°C。这使得它可以分析一些沸点较高的化合物。
无液相流失：因为它本身就是固态的聚合物填料，而不是涂覆了液相的载体，所以在操作过程中不存在固定液流失的问题。这使得它在使用对流失敏感检测器（如ECD、MS）时背景信号更稳定。
适用于极性物质：是分析水、醇、脂肪酸、乙二醇、胺类、醛类、酮类等极性化合物的经典选择。

主要应用
Chromosorb 101 特别擅长分离以下类型的化合物：
水（H₂O）：可以在其他有机组分出峰之前先出峰，且峰形尖锐，非常适合有机物中水分的分析。
游离脂肪酸（C₁-C₅）：如甲酸、乙酸、丙酸等。
醇类：如甲醇、乙醇、丙醇、二醇等。
醛类和酮类：如甲醛、乙醛、丙酮等。
胺类和腈类。
一些活性气体，如硫化氢（H₂S）、二氧化硫（SO₂）。
经典应用示例：发酵液或酒精饮料中的挥发性有机物（杂醇油）分析。

 

浩瀚色谱（山东）应用技术开发有限公司，研制的《Chromosorb 101填充色谱柱应用PE580》：

 

适合分析极性化合物，如醇、酸、胺等，因为这些化合物在惰性表面上不易发生拖尾现象。

 

名称：填充柱

材质：不锈钢

型号：Chromosorb 103

规格：4m*3mm，60-80目

zui高使用温度：240°C

应用：水、醇、脂肪酸、乙二醇、胺类、醛类、酮类

 

 

应用：

安捷伦490在线/便携，4890,5890,6890,7820,7890,8860,8890

岛津GC-14C，GC-2010，GC-2014，GC-2030

赛默飞1310,1300,1610,1600

瓦里安3800系列

布鲁克PE580,590,680,690

磐诺A90，福立GC9790，华爱，天瑞，天美，GC7900，川仪，北分瑞利，东西电子，鲁南瑞虹，捷岛/科晓
 

Chemical composition: Chromosorb 101 is a copolymer of styrene divinylbenzene. Diethylbenzene (DVB) is used as a crosslinking agent to connect linear polystyrene chains into a three-dimensional network structure, thereby forming porous microspheres with mechanical strength and thermal stability.
Polarity: It is a non-polar stationary phase, and its separation mechanism is mainly based on van der Waals forces and hydrophobic interactions.
Physical form: usually small white or milky white particles with uniform particle size (such as 80-100 mesh, 100-120 mesh, etc.) for filling into chromatographic columns.
Surface characteristics: Compared to diatomaceous earth carriers such as Chromosorb W, its surface is inert and has almost no active adsorption sites. This makes it particularly suitable for analyzing polar compounds such as alcohols, acids, amines, etc., as these compounds are less prone to tailing on inert surfaces.
Suitable for polar substances: It is a classic choice for analyzing polar compounds such as water, alcohols, fatty acids, ethylene glycol, amines, aldehydes, ketones, etc.
Main applications
Chromosorb 101 is particularly adept at separating the following types of compounds:
Water (H ₂ O): It can peak before other organic compounds, and the peak shape is sharp, which is very suitable for the analysis of moisture in organic matter.
Free fatty acids (C ₁ - C ₅): such as formic acid, acetic acid, propionic acid, etc.
Alcohols: such as methanol, ethanol, propanol, diols, etc.
Aldehydes and ketones: such as formaldehyde, acetaldehyde, acetone, etc.
Amines and nitriles.
Some reactive gases, such as hydrogen sulfide (H ₂ S) and sulfur dioxide (SO ₂).
Classic application example: Analysis of volatile organic compounds (fusel oil) in fermentation broth or alcoholic beverages.
Haohan Chromatography (Shandong) Application Technology Development Co., Ltd. has developed the "Chromosorb 101 Filled Chromatography Column Application PE580":
Suitable for analyzing polar compounds such as alcohols, acids, amines, etc., as these compounds are less prone to tailing on inert surfaces.
Name: Filler Column
Material: Stainless steel
Model: Chromosorb 103
Specification: 4m * 3mm, 60-80 mesh
Maximum operating temperature: 240 ° C
Applications: Water, alcohols, fatty acids, ethylene glycol, amines, aldehydes, ketones
Application:
Agilent 490 online/portable, 48905890689088608890
Shimadzu GC-14C, GC-2010, GC-2014, GC-2030
Thermo Fisher Scientific 1310130016101600